實驗室的求生守則
Lab Survival Manual剛來到實驗室不曉得實驗室運作的規則？ 第一次 PCR 什麼東西都沒 P 到？ 細胞怎麼養就是會汙染？ 照著 Kit 的 Protocol 做，卻怎麼都做不出來？ 不要緊，讓 Norton 和 Steve (a.k.a. Worta) 來罩你！ 我們兩個是在生物實驗室念書的博班生，雖然沒有通天的本領，但也還沒忘記第一次拿起 Pipette 的感動/恐懼！ 希望藉由這個 Podcast 頻道，讓大家能速速了解實驗以及在實驗室生活的各種眉角：D 如果想發問或是跟我們聊天，歡迎寄信到 intermemebrain.space@gmail.com --
| Publishes | Monthly | Episodes | 12 | Founded | 4 years ago |
|---|---|---|---|---|---|
| Language |  ZH-TW | Categories | Life SciencesScience | ||
Listen to this Podcast
Latest Episodes
基因克隆（cloning）大長篇即將來到尾聲，我們要用序列比對（alignment）來「對答案」、看到底有沒有成功得到想要的序列；然而這些作為參考標準的序列到底怎麼來的呢？人類基因組定序從上世紀末到 2022 年的重大突破，到底又是用什麼樣的技術完成的？本集邀請到攻讀生物資訊學門的好朋友－－嚇淋－－來跟我們一起聊聊怎麼把人類的整個基因庫給組裝起來！
By the way, Norton 的另一檔節目已經發布第一集，歡迎對實驗動物福祉有興趣的朋友前往收聽！
南加 Bio 咖聊新聞 By S... more
這集介紹了 RNA 和如何從細胞中抽取它們，怎麼跑膠、定量，以及轉換成 cDNA 時的一些小細節。
Norton 在另一個頻道的新節目正在緊鑼密鼓地準備中，歡迎搶先訂閱！
南加 Bio 咖聊新聞 By SoCal TBA
PCR 後跑了膠發現沒 band、好多條 band、各種奇怪結果？本集就介紹給你幾個絕招－－Gradient、Touch-down、DMSO 等各式各樣調整的技巧，讓你事半功倍！ 提問用信箱：intermemebrain.space@gmail.com
大家新年快樂！剛經歷了人生轉折(?)的兩位主持人，在本集準備了有關聚合酶連鎖反應（PCR）實驗的主題！PCR 是實驗室家家戶戶必備的一項分子生物學技術，也是許許多多的新手在 cloning 時容易卡關的瓶頸。若你正好為此苦惱，歡迎進來聽聽看 Norton 和 Worta 仔細討論引子（primer）設計需要考慮的多種面向，以及詳解 PCR 的材料和步驟。本集開始，Worta 音質大提升，Norton 也嘗試自己純手工混音，歡迎提供聲音方面的意見：D
本集提及的實用工具：Snapgene、Pr... more
微生物操作知識以及嘴砲的愉悅融合！本集邀請到大尾的大尾來跟我們一起聊聊塗菌方法、培養環境、抗生素的選用，以及簡介生物安全等級。
經過忙碌的三週，我們回來啦：D
雖然上次提過養菌，但這兩集我們要來討論怎麼製作培養基，還有無菌操作的基本知識（& 姿勢），讓你再也不怕養菌汙染！
書報討論、專題討論......這些令人聞風喪膽的課就已經夠讓人吃不消，進了實驗室又時不時需要在 meeting 的時候報 paper，到底要怎麼辦才不會被老闆電爆？本集討論如何挑選並快速讀懂 paper，也提供報告時的小技巧和需要避開的常見問題。
只要有了這些酵素，plasmids 的序列也能 Ctrl+X、Ctrl+V！
本集教你如何挑選限制酶（restriction enzyme）、接合酶（ligase）的使用、如何進行轉形（transformation）將 plasmids 送進細菌裡面，以及有什麼菌株可以用來表現蛋白質或其他用途。
Key Facts
Top Life Sciences Podcasts
Audience Metrics
Listeners, social reach, demographics and more for this podcast.
| Gender Skew | Location | Interests | |||
|---|---|---|---|---|---|
| Professions | Age Range | Household Income | |||
| Social Media Reach | |||||
Frequently Asked Questions About 實驗室的求生守則
Where can I find podcast stats for 實驗室的求生守則?
Rephonic provides a wide range of podcast stats for 實驗室的求生守則. We scanned the web and collated all of the information that we could find in our comprehensive podcast database. See how many people listen to 實驗室的求生守則 and access YouTube viewership numbers, download stats, audience demographics, chart rankings, ratings, reviews and more.
How many listeners does 實驗室的求生守則 get?
Rephonic provides a full set of podcast information for three million podcasts, including the number of listeners. View further listenership figures for 實驗室的求生守則, including podcast download numbers and subscriber numbers, so you can make better decisions about which podcasts to sponsor or be a guest on. You will need to upgrade your account to access this premium data.
What are the audience demographics for 實驗室的求生守則?
Rephonic provides comprehensive predictive audience data for 實驗室的求生守則, including gender skew, age, country, political leaning, income, professions, education level, and interests. You can access these listener demographics by upgrading your account.
How many subscribers and views does 實驗室的求生守則 have?
To see how many followers or subscribers 實驗室的求生守則 has on Spotify and other platforms such as Castbox and Podcast Addict, simply upgrade your account. You'll also find viewership figures for their YouTube channel if they have one.
How many episodes of 實驗室的求生守則 are there?
實驗室的求生守則 launched 4 years ago and published 12 episodes to date. You can find more information about this podcast including rankings, audience demographics and engagement in our podcast database.
How do I contact 實驗室的求生守則?
Our systems regularly scour the web to find email addresses and social media links for this podcast. We scanned the web and collated all of the contact information that we could find in our podcast database. But in the unlikely event that you can't find what you're looking for, our concierge service lets you request our research team to source better contacts for you.
Where can I see ratings and reviews for 實驗室的求生守則?
Rephonic pulls ratings and reviews for 實驗室的求生守則 from multiple sources, including Spotify, Apple Podcasts, Castbox, and Podcast Addict.
View all the reviews in one place instead of visiting each platform individually and use this information to decide if a show is worth pitching or not.
How do I access podcast episode transcripts for 實驗室的求生守則?
Rephonic provides full transcripts for episodes of 實驗室的求生守則. Search within each transcript for your keywords, whether they be topics, brands or people, and figure out if it's worth pitching as a guest or sponsor. You can even set-up alerts to get notified when your keywords are mentioned.