Rephonic
Artwork for Absolute Gene-ius
Digital PCR
Thermo Fisher Scientific
QPCR
Alzheimer's Disease
Quantstudio Absoluteq DPCR
Adeno-Associated Virus
Epigenetics
Edna
Microvesicles
Saliva
Hiv-2
Downy Mildew Of Grapevine
Plasmopara Viticola
Grape Clades (P. Viticola Cryptic Species)
Real-Time PCR
Matica Biotechnology
Contract Development and Manufacturing Organization
Sequencing
Liquid Biopsy
Next-Generation Sequencing

Inspiring stories and advice from dPCR gene-iuses.

PublishesTwice monthlyEpisodes42Founded3 years ago
Number of ListenersCategories
ScienceLife SciencesChemistry

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Artwork for Absolute Gene-ius

Latest Episodes

In this episode, Michael Stump and Matteo Franco from ProtaGene explore the growing importance of integration site analysis (ISA) in cell and gene therapy. As viral vectors like lentivirus and AAV become more widely used, understanding where and how ... more

PCR may be decades old, but it is still one of the most versatile tools in modern molecular biology.

In this crossover episode, Absolute Gene-ius welcomes Steve Lewis, host of Speaking of Mol Bio and Director of Product Management at Thermo Fisher S... more

Dr. Robert Philibert joins the show to unpack the fascinating world of epigenetics, where environmental factors like smoking, alcohol, and lifestyle reshape how genes are expressed. He explains how methylation, histone modifications, and chromatin st... more

Multiplexing is reshaping how scientists think about PCR experiments, turning single-target workflows into powerful, multi-dimensional analyses.

In this Science Snapshot, we revisit standout moments from past episodes to explore how multiplexing is ... more

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Recent Guests

Steve Lewis
Host of Speaking of Mol Bio, Senior Product Manager, Molecular Biology, Thermo Fisher Scientific
Thermo Fisher Scientific
Episode: From brewing to bioprocessing – Steve Lewis on molecular biology trends
Robert Philibert
Professor of Psychiatry at the University of Iowa
University of Iowa; Behavioral Diagnostics
Episode: Epigenetics unlocked – how behavior shapes biology
Fiona Connolly
Platform innovation and automation scientist at BitBio
BitBio
Episode: Finding the cheat codes to cell identity
Daniel Mitchell
Head of Analytical Development and Quality Control at Matica Biotechnology
Matica Biotechnology
Episode: Almost a virus – how AAV powers modern gene therapy
Kathie Sollweck
Senior Medical Affairs Manager, Oncology at Thermo Fisher
Thermo Fisher Scientific
Episode: How sequencing and PCR work together in oncology research
Lexi Heger
PhD candidate in Plant Pathology and Molecular Plant Sciences at Michigan State University
Michigan State University
Episode: Tracking grape pathogens from air to vine
Jesús Mingorance
Researcher in Microbiology and antibiotic resistance dynamics
Hospital Universitario La Paz, Madrid, Spain
Episode: Digital PCR and the fight against HIV-2 and superbugs
Arianna Arbona
PhD student in Biochemistry and Neuroscience
Universidad Pablo de Olavide, Sevilla, Spain
Episode: Biomarkers in motion ― detecting Alzheimer's through saliva
Brian Bahder
Associate Professor of Insect Vector Ecology
University of Florida
Episode: Python problems – digital PCR takes on the Everglades

Hosts

Jordan Ruggeri
Host of Absolute Gene-ius; Thermo Fisher Scientific
Lisa Crawford
Co-host of Absolute Gene-ius (and related series)
Christina Bowens
Co-host on Absolute Gene-ius

Chart Rankings

How this podcast ranks in the Apple Podcasts, Spotify and YouTube charts.

Talking Points

Recent interactions between the hosts and their guests.

Season 4 – new voices, new discoveries
Q: Are you ready for yours?
Lisa responds enthusiastically, sharing her readiness and eagerness to contribute as a co-host.
Digital PCR and the fight against HIV-2 and superbugs
Q: How did the HIV-2 work influence or align with the broader work in clinical diagnostics, especially in relation to other pathogens like CMV or hepatitis B?
The episode discusses applying QPCR and DPCR across various pathogens to improve diagnostic sensitivity and quantification, emphasizing the need for robust standards, flexible designs, and the potential to replace or complement existing methods in clinical laboratories.
Digital PCR and the fight against HIV-2 and superbugs
Q: What were the aims of the HIV-2 publication you discussed, and what were you trying to accomplish?
The team aimed to evaluate ultrasensitive/digital PCR for HIV-2 detection and quantification, including integration of the virus into proviral DNA and assessing how samples with low or undetectable viral loads could still be meaningfully analyzed, comparing with NASBA and other assays.
Finding the cheat codes to cell identity
Q: How does your platform ensure consistency across batches and clones?
By using digital PCR to quantify transgene copy numbers and expression fingerprints, and by deterministic programming to standardize how factors are delivered and switched on, ensuring reproducible cell types.
Finding the cheat codes to cell identity
Q: What exactly are IPSCs and why are they important?
IPSCs are adult cells reprogrammed back into a pluripotent state, capable of becoming any cell type, which enables disease modeling, drug discovery, and cell therapies without relying on embryonic sources.

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Frequently Asked Questions About Absolute Gene-ius

What is Absolute Gene-ius about and what kind of topics does it cover?

This show centers on the practical uses of digital and real-time PCR, often bridging bench science with real-world workflows in biotech, clinical research, and environmental monitoring. Episodes typically feature subject-matter experts and practitioners who break down advanced techniques (digital PCR, qPCR, sequencing) and their applications in oncology, infectious disease, neurodegeneration, stem cell programming, and plant pathology, often weaving in career journeys and industry perspectives. A notable strength is the blend of deeply technical content with accessible storytelling, personal anecdotes, and behind-the-scenes insights from collaborations with major life-science suppliers, which can help listeners evaluate cutting-edge tools, ... more

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How many episodes of Absolute Gene-ius are there?

Absolute Gene-ius launched 3 years ago and published 42 episodes to date. You can find more information about this podcast including rankings, audience demographics and engagement in our podcast database.

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What guests have appeared on Absolute Gene-ius?

Recent guests on Absolute Gene-ius include:

1. Steve Lewis
2. Robert Philibert
3. Fiona Connolly
4. Daniel Mitchell
5. Kathie Sollweck
6. Lexi Heger
7. Jesús Mingorance
8. Arianna Arbona

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