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實驗室的求生守則

Lab Survival Manual

剛來到實驗室不曉得實驗室運作的規則? 第一次 PCR 什麼東西都沒 P 到? 細胞怎麼養就是會汙染? 照著 Kit 的 Protocol 做,卻怎麼都做不出來? 不要緊,讓 Norton 和 Steve (a.k.a. Worta) 來罩你! 我們兩個是在生物實驗室念書的博班生,雖然沒有通天的本領,但也還沒忘記第一次拿起 Pipette 的感動/恐懼! 希望藉由這個 Podcast 頻道,讓大家能速速了解實驗以及在實驗室生活的各種眉角:D 如果想發問或是跟我們聊天,歡迎寄信到 intermemebrain.space@gmail.com -- Support this podcast: https://podcasters.spotify.com/pod/show/lab-survival-manual/support

PublishesWeeklyEpisodes12Founded3 years ago
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ScienceLife Sciences

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Artwork for 實驗室的求生守則

Latest Episodes

基因克隆(cloning)大長篇即將來到尾聲,我們要用序列比對(alignment)來「對答案」、看到底有沒有成功得到想要的序列;然而這些作為參考標準的序列到底怎麼來的呢?人類基因組定序從上世紀末到 2022 年的重大突破,到底又是用什麼樣的技術完成的?本集邀請到攻讀生物資訊學門的好朋友--嚇淋--來跟我們一起聊聊怎麼把人類的整個基因庫給組裝起來! more

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2 years ago

這集介紹了 RNA 和如何從細胞中抽取它們,怎麼跑膠、定量,以及轉換成 cDNA 時的一些小細節。 more

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2 years ago

PCR 後跑了膠發現沒 band、好多條 band、各種奇怪結果?本集就介紹給你幾個絕招--Gradient、Touch-down、DMSO 等各式各樣調整的技巧,讓你事半功倍! 提問用信箱:intermemebrain.space@gmail.com --- Send in a voice message: https://podcasters.spotify.com/pod/show/lab-survival-manual/message Support this podcast: http... more

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2 years ago

大家新年快樂!剛經歷了人生轉折(?)的兩位主持人,在本集準備了有關聚合酶連鎖反應(PCR)實驗的主題!PCR 是實驗室家家戶戶必備的一項分子生物學技術,也是許許多多的新手在 cloning 時容易卡關的瓶頸。若你正好為此苦惱,歡迎進來聽聽看 Norton 和 Worta 仔細討論引子(primer)設計需要考慮的多種面向,以及詳解 PCR 的材料和步驟。本集開始,Worta 音質大提升,Norton 也嘗試自己純手工混音,歡迎提供聲音方面的意見:D more

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2 years ago

微生物操作知識以及嘴砲的愉悅融合!本集邀請到大尾的大尾來跟我們一起聊聊塗菌方法、培養環境、抗生素的選用,以及簡介生物安全等級。 --- Send in a voice message: https://podcasters.spotify.com/pod/show/lab-survival-manual/message Support this podcast: https://podcasters.spotify.com/pod/show/lab-survival-manual/support... more

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2 years ago

經過忙碌的三週,我們回來啦:D more

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2 years ago

書報討論、專題討論......這些令人聞風喪膽的課就已經夠讓人吃不消,進了實驗室又時不時需要在 meeting 的時候報 paper,到底要怎麼辦才不會被老闆電爆?本集討論如何挑選並快速讀懂 paper,也提供報告時的小技巧和需要避開的常見問題。 --- Send in a voice message: https://podcasters.spotify.com/pod/show/lab-survival-manual/message Support this podcast: https:/... more

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3 years ago

只要有了這些酵素,plasmids 的序列也能 Ctrl+X、Ctrl+V! more

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3 years ago

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